Incorporation of silver nanoparticles on the surface of orthodontic microimplants to achieve antimicrobial properties
Venugopal Adith, Muthuchamy Nallal, Tejani Harsh, Anantha Iyengar Gopalan, 이광필, 이헌진, 경희문,
소속 상세정보
( Venugopal Adith ) - Kyungpook National University School of Dentistry Department of Orthodontics
( Muthuchamy Nallal ) - Kyungpook National University Department of Chemistry Education
( Tejani Harsh ) - Kyungpook National University School of Dentistry Department of Orthodontics
( Anantha Iyengar Gopalan ) - Kyungpook National University Research Institute of Advanced Energy Technology
이광필 ( Lee Kwang-Pill ) - Kyungpook National University Department of Chemistry Education
이헌진 ( Lee Heon-Jin ) - Kyungpook National University Department of Oral Microbiology and Immunology
경희문 ( Kyung Hee-Moon ) - Kyungpook National University School of Dentistry Department of Orthodontics
KMID : 0361920170470010003
Abstract
Objective: Microbial aggregation around dental implants can lead to loss/loosening of the implants. This study was aimed at surface treating titanium microimplants with silver nanoparticles (AgNPs) to achieve antibacterial properties.
Methods: AgNP-modified titanium microimplants (Ti-nAg) were prepared using two methods. The first method involved coating the microimplants with regular AgNPs (Ti-AgNP) and the second involved coating them with a AgNP-coated biopolymer (Ti-BP-AgNP). The topologies, microstructures, and chemical compositions of the surfaces of the Ti-nAg were characterized by scanning electron microscopy (SEM) equipped with energydispersive spectrometer (EDS) and X-ray photoelectron spectroscopy (XPS). Disk diffusion tests using Streptococcus mutans, Streptococcus sanguinis, and Aggregatibacter actinomycetemcomitans were performed to test the antibacterial activity of the Ti-nAg microimplants.
Results: SEM revealed that only a meager amount of AgNPs was sparsely deposited on the Ti-AgNP surface with the first method, while a layer of AgNP-coated biopolymer extended along the Ti-BP-AgNP surface in the second method. The diameters of the coated nanoparticles were in the range of 10 to 30 nm. EDS revealed 1.05 atomic % of Ag on the surface of the Ti-AgNP and an astounding 21.2 atomic % on the surface of the Ti-BP-AgNP. XPS confirmed the metallic state of silver on the Ti-BP-AgNP surface. After 24 hours of incubation, clear zones of inhibition were seen around the Ti-BP-AgNP microimplants in all three test bacterial culture plates, whereas no antibacterial effect was observed with the Ti-AgNP microimplants.
Conclusions: Titanium microimplants modified with Ti-BP-AgNP exhibit excellent antibacterial properties, making them a promising implantable biomaterial.
키워드
Nanosilver; Microimplant; Antimicrobial
원문 및 링크아웃 정보
등재저널 정보